Shythdf3
WebMar 10, 2024 · e, f qRT-PCR and immunoblot analysis of the indicated mRNA and protein in HTR8/SVneo transfected with shYTHDF3 for 72 h. g, i RNA lifetime for IGF1R in … WebThe objectives are to identify expression profiles that are specific to shYTHDF3 breast cancer cell to identify new the signaling pathway of YTHDF3 for brain metastasis of …
Shythdf3
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WebOct 4, 2024 · a, b mCherry-GFP-LC3 was transfected into shNS and shYTHDF3 MEFs, and autophagosome (yellow) and autolysosome (red) formation was examined. Scale bar, 20 … WebMar 7, 2024 · Abstract. N 6-Methyladenosine (m 6 A) is a dynamic mRNA modification which regulates protein expression in various posttranscriptional levels. Functional studies of m …
WebFigure Legend Snippet: a , b mCherry-GFP-LC3 was transfected into shNS and shYTHDF3 MEFs, and autophagosome (yellow) and autolysosome (red) formation was examined. Scale bar, 20 μm. c , d Endogenous ULK1, ATG13, ATG14, and DFCP1 puncta in shNS and shYTHDF3 MEFs were immunostained after nutrient deficiency, visualized with confocal … WebOct 29, 2024 · Brain metastasis is a major cause of cancer mortality, but its molecular mechanisms are severely understudied. In addition, little is known regarding the role of m 6 A reader YTHDF3 in human diseases. Here we show that YTHDF3 overexpression clinically correlates with brain metastases in breast cancer patients.
WebDec 6, 2024 · Meanwhile, two short hairpin RNAs (shYTHDF3–1 and shYTHDF3–2) were transfected into Huh7 and SNU449 cells to construct YTHDF3-stably-knocked down cell … WebFigure Legend Snippet: a , b mCherry-GFP-LC3 was transfected into shNS and shYTHDF3 MEFs, and autophagosome (yellow) and autolysosome (red) formation was examined. Scale bar, 20 μm. c , d Endogenous ULK1, ATG13, ATG14, and DFCP1 puncta in shNS and shYTHDF3 MEFs were immunostained after nutrient deficiency, visualized with confocal …
WebJun 28, 2024 · Scale bar, 10 μm (n=3 independent experiments); c. Quantification of YTHDF3 protein expression by immuno-staining in shScram and shYTHDF3 cells (Scram, sh1, and sh2).
WebMDA-MB231Br cells were transduced with shControl and shYTHDF3 lentivirus and selected by puromycin. Growth protocol: MDA-MB-231Br cells were grown in DMEM medium with 10% FBS and 1% penicillin-streptomycin. Cells were grown in a 5% CO2 cell culture incubator at 37℃. Extracted molecule: total RNA: Extraction protocol list of hiking gearWebMDA-MB-231 shYTHDF3 1: Sample type: RNA : Source name: MDA-MB-231 shYTHDF3: Organism: Homo sapiens: Characteristics: cell line: MDA-MB-231Br cell type: Breast Cancer Cell Line genotype/variation: YTHDF3 knockdown: Treatment protocol: MDA-MB231Br cells were transduced with shControl and shYTHDF3 lentivirus and selected by puromycin. imareefer.comWebOct 29, 2024 · Chang et al. report that YTHDF3 overexpression associates with breast cancer brain metastasis and confers poor survival. Mechanistically, YTHDF3 enhances … ima realty willimantic ctWebFeb 1, 2024 · N 6-methyladenosine (m 6 A) is a reversible modification in mRNA and has been shown to regulate processing, translation and decay of mRNA. However, the roles of m 6 A modification in neuronal development are still not known. Here, we found that the m 6 A eraser FTO is enriched in axons and can be locally translated. Axon-specific inhibition of … ima red bank covid testingWebDec 6, 2024 · Europe PMC is an archive of life sciences journal literature. im a red flagWebDec 9, 2024 · Background METTL3 is an RNA methyltransferase that mediates m6A modification and is implicated in mRNA biogenesis, decay, and translation. However, the biomechanism through which METTL3 regulates MALAT1-miR-1914-3p-YAP axis activity to induce NSCLC drug resistance and metastasis is not very clear. Methods The expression … ima recreation uwWebf Left: Migration (e) and invasion (f) analysis of HTR8/SVneo cells stably expressing control shRNA (shControl) vs. shRNA targeting YTHDF3 (shYTHDF3) by Transwell assay. … im a real spring chicken and im having a ball