Phis2图谱
Webb2.制备感受态并将pHis2-DNA转化进入感受态细胞; 3.SD/-Trp平板检测转化结果。 二、自激活检测 1.制备不同3-AT浓度梯度的SD/-His/-Trp平板,3-AT的浓度一般设定在50-100mM … WebbpHIS2质粒. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One-Hybrid Library Construction & …
Phis2图谱
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Webb2 aug. 2024 · 酵母单杂交 (yeast one hybrid)技术是体外分析DNA与细胞内蛋白质相互作用的一种方法,通过对酵母细胞内报告基因表达状况的分析,来鉴别DNA结合位点并发现潜在的结合蛋白基因,或对DNA结合位点进行分析。. 运用此技术,能筛选到与DNA结合的蛋白质,并可直接从 ... http://www.biofeng.com/zaiti/jiaomu/pHis2.html
Webb1 juni 2024 · Clone the products of the PCR reactions carried out in step 3 into the pHIS2 T-vector obtained in step 5. The T-A ligation system includes 0.2 μg of T-vector of pHIS2, 0.5 μL of PCR products (Y1 + Y3 and Y2 + Y3), 1 μL of 10× ligation buffer, 3 U of T4 ligase, and PEG6000 at final concentration of 10% (w/v). http://www.vectorhub.cn/read-plasmid-profiles.html
WebbVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community …
Webb载体质粒图谱和多克隆位点信息 载体名称 pESC-‐Leu Agilent 安捷伦 pESC-‐Leu, pESC Leu 酿酒酵母蛋白表达载体 高拷贝 半乳糖 GAL1和GAL10 多克隆位点,限制性内切酶 7758 bp sia right to workWebbpHis2载体价格1000元,pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文,载体质粒抗性为Kanamycin,质粒大小为7207bp。pHis2是酵母单杂交载体的pHis2。 the peoplease grouphttp://www.biovector.net/product/425328.html the people are the heroes nowWebbxho1和sac1的酶切位点是什么? 它识别的特定基因是体内3广a泛存在的siRNA和miRNA前体。Dicer酶的PAZ结构域特异性地识别 0’端有 5个j碱基突出的 dsRNA,之w后由RNase III结构域剪切8生成siRNA或microRNA。 2011-10-27 8:58:59 the people are talking nicki minajWebb10 nov. 2015 · 植物表达载体 pcambia1301(EHA105). 可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真核表达及纯,pQE 载体,原核表达载体,克隆载体 ,毕赤酵母表达载体,毕赤酵母宿主菌,酿酒酵母表达载体 ,酿酒 ... siarm italyWebbThe entire Matchmaker screening process consists of the following steps: • Step 1. Perform control experiments • Step 2. Clone your target sequence (bait) and optimize 3-AT • Step 3. Construct and screen library by cotransformation and in vivorecombination • Step 4. Confirm and interpret results GAL4 AD Library protein Tminimal promoterHIS3 sia rouh phinWebb1、文库质量评估:理论+涂板评价. 交付材料:反馈质量评估结果. 2、载体构建:基因合成诱饵基因 构建Phis2载体. 交付材料:含诱饵基因的质粒. 3、启动子背景筛查:Binding seq基因启动子的背景筛查. 交付材料:阳性克隆测序结果 word项目报告. 4、文库筛选:诱 … siarkol extra 80 wp