WebDroplet digital PCR (ddPCR) assays, Supermix for probes, DG8TM cartridges and Droplet Generation Oil were obtained from Bio-Rad Laboratories Ltd., Hertfordshire, UK. Deionized water, sodium chloride, phosphate-buffered saline (PBS), sodium nitrate, 4 … WebJun 4, 2024 · Then, 20 μl of the ddPCR reactions along with 70 μl of droplet generation oil for EvaGreen (Bio-Rad) were loaded onto an 8-channel DG8TM cartridge for droplet generation in the QX200 Droplet Generator (Bio-Rad).
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